Abstract
Abnormal changes of intracellular microviscosity are associated with a series of pathologies and diseases. Therefore, monitoring viscosity at cellular and subcellular levels is important for pathological research. Fluorescent molecular rotors (FMRs) have recently been developed to detect viscosity through a linear correlation between fluorescence intensity or lifetime and viscosity. Recently, 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (boron dipyrrins or BODIPY) derivatives have been widely used to build FMRs for viscosity probes due to their high rotational ability of the rotor and potentially high brightness. In this minireview, functionalized BODIPYs as FMRs for viscosity detection were collected, analyzed and summarized.
Highlights
The viscosity of cells is an important parameter of the cellular microenvironment, which influences the interaction and transport of biological molecules and signals in living cells (Minton, 2001)
The fluorescence lifetime imaging (FLIM) using 3 was carried out to study intracellular viscosity, and the results indicated that the average viscosity of SK-OV-3 cells was 140 ± 40 cP
The results showed that the rotational correlation time (θ ) of BODIPY 3 increased linearly with solvent viscosity (Figure 1G)
Summary
The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-Based Materials, School of Chemistry and Materials Science, Anhui Normal University, Wuhu, China. Monitoring viscosity at cellular and subcellular levels is important for pathological research. Fluorescent molecular rotors (FMRs) have recently been developed to detect viscosity through a linear correlation between fluorescence intensity or lifetime and viscosity. 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (boron dipyrrins or BODIPY) derivatives have been widely used to build FMRs for viscosity probes due to their high rotational ability of the rotor and potentially high brightness. In this minireview, functionalized BODIPYs as FMRs for viscosity detection were collected, analyzed and summarized
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