Abstract
We propose a polymeric nanofilter biointerface with a multilayer that suppresses nonspecific signals caused by interfering species such as proteins and small biomolecules for potentiometric biosensors. In a previous work, we successfully prevented small biomolecules from reacting with the Au electrode using a polymeric nanofilter with a single polymeric layer, resulting in the suppression of nonspecific signals in an extended-Au-gate field-effect transistor (FET). In this study, poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) is additionally employed as an antifouling nanofilter layer to suppress the nonspecific adsorption of proteins on the Au gate surface. We precisely graft the polymeric nanofilter on the Au electrode via diazonium chemistry and activators regenerated by electron transfer surface-initiated atom transfer radical polymerization (ARGET SI-ATRP). By impedimetric analysis, we found that the charge transfer resistance (R CT) of the Au electrode with the PMPC-based nanofilter hardly changes even when human serum albumin (HSA) is added onto the electrode. That is, the PMPC-based nanofilter effectively contributes to the prevention of the nonspecific adsorption of proteins such as HSA.
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