Abstract
The homogeneous nanocomposites of 1-pyrene butyric acid modified nitrogen-doped carbon nanotubes (PBA/NCNTs) are synthesized for the immobilization of enzymes and glucose biosensing. Through the aqueous carbodiimide coupling chemistry, glucose oxidase (GOD) is covalently immobilized on the surface of the as-prepared PBA/NCNTs with high enzyme loading (1.986 nmol · cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-2</sup> ). Fast direct electron transfer between GOD and the electrode is observed with the electron transfer rate constant (ks) of 2.43 s <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> . Besides, the GOD/PBA/NCNTs-based biosensor exhibits a linear response to glucose concentrations ranging from 2 μM to 0.2 mM with the detection limit of 1.33 μM and the high sensitivity of 179.76 μA·mM <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> · cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-2</sup> . Furthermore, this biosensor is also applied to detect glucose in the human serum samples with high accuracy. These results demonstrate that the PBA/NCNTs nanocomposites can be a promising platform for immobilizing biomolecules and biosensing application.
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