Abstract
Traditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. Therefore, new methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods can connect high sensitive sensing systems with biological requirements. One approach is to functionalize an extended-gate field-effect transistor’s (EGFET) sensing area with modified porphyrins containing two different linkers. One linker connects the electrode surface with the porphyrin. The other linker bonds bacteria on the functional layer through a specific peptide chain. The negative charge on the surface of the cells regulates the surface potential which has an impact on the electrical behavior of the EGFET. The attendance of attached bacteria on the functionalized sensing area could successfully be detected.
Highlights
Traditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility
The influence of bacteria on the electrical behavior of the developed extended-gate field-effect transistor (EGFET) are discussed with results recorded by two operation modes
This work focuses on the detection of bacteria by the developed EGFET
Summary
Traditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. New methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods can connect high sensitive sensing systems with biological requirements. One approach is to functionalize an extended-gate field-effect transistor’s (EGFET) sensing area with modified porphyrins containing two different linkers. The attendance of attached bacteria on the functionalized sensing area could successfully be detected. Bacteria are often seen as pathogenic organisms but essential functions, for instance in healthcare and food safety, cannot be solved without microorganisms[1,2]. Biosensors based on bioelectronics have a fast response, improve the sensitivity, and even reduce the sample volume drastically compared to traditional sensing technologies[6,7]. The sensing area of an EGFET is functionalized by modified porphyrins and further covered with a liquid containing bacteria. Our developed EGFET as biosensor for the detection of bonded bacteria is introduced.
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