Abstract
3D materials supporting cell adhesion, infiltration and proliferation are crucial for bone tissue engineering. In the current study we combined PCL fibers prepared using centrifugal spinning with adhered liposomes filled with platelet lysate as a natural source of growth factors. The scaffold was seeded with MG-63 cells and tested in vitro as a potential drug delivery system for bone tissue engineering.
Highlights
Nowadays 3D materials supporting cell adhesion, infiltration and proliferation are needed in tissue engineering applications
Nanofibers and microfibers are often used in tissue engineering, because they mimic the natural extracellular matrix (ECM) and enhance cell adhesion and proliferation [2]
Osteogenic differentiation was detected as synthesis and production of proteins typical for bone ECM, type I collagen and osteocalcin using real-time PCR (Light Cycler 480 II; Roche, Switzerland) and immunofluorescence staining
Summary
Nowadays 3D materials supporting cell adhesion, infiltration and proliferation are needed in tissue engineering applications. These materials should serve as a scaffold, and promote cell ingrowth and differentiation into the required cell type. Nanofibers and microfibers are often used in tissue engineering, because they mimic the natural extracellular matrix (ECM) and enhance cell adhesion and proliferation [2]. Centrifugal spinning uses centrifugal force to produce ultrafine fibers from melts and solutions [3], [4]. This method gives rise to fibers with submicron diameters and fluffy 3D structure. As a natural source of cytokines and growth factors the platelet lysate (PL) was used to boost the growth of MG-63 cells
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