Functionalization and self-assembly of DNA bidimensional arrays.

Alejandra V Garibotti,Sónia Pérez-Rentero,Ramon Eritja

doi:10.3390/ijms12095641

Abstract

Oligonucleotides carrying amino, thiol groups, as well as fluorescein, c-myc peptide sequence and nanogold at internal positions were prepared and used for the assembly of bidimensional DNA arrays.

Highlights

In recent years the use of the self-assembly properties of DNA to generate addressable nanostructures has been extensively investigated [1,2,3]
We focused on the A-B* tile system described by Winfree et al [6] which comprises two DNA tiles, A and B*
Tile B* has two loops protruding out of the plane of the DNA lattice in opposite directions. These two loops were used as topographic labels to indicate the position of tile B* on the DNA lattice during the process of visualization by atomic force microscopy (AFM)

Summary

Introduction

In recent years the use of the self-assembly properties of DNA to generate addressable nanostructures has been extensively investigated [1,2,3]. DNA has many physical and chemical properties that make it a useful molecule for the assembly of nanostructures [1,2,3]. The solid-phase synthesis and the PCR amplification methods have made long and complex DNA molecules readily available in abundant quantities [4]. The relatively high physicochemical stability makes DNA easier to handle in normal laboratory conditions [1,2,3,4]. A remarkable development in this field was the use of stable DNA Holliday junctions with addressable sticky ends to form two-dimensional DNA crystals [6].

Objectives

Results

Conclusion