Abstract
Previous studies have shown that functionalization of hydrogel membranes with metal affinity ligands increases the separation potential of the membranes for purification of biological systems. To achieve separation, histidines that are exposed on the proteins surface are attracted and attach to the metal ion within the IMA membrane matrix. The effectiveness of these membranes depends on several factors including, but not limited to, type of chelating group, the metal ion, and solution buffer. This paper is concerned with crosslinking of poly (vinyl) alcohol (PVA) with varied degrees of crosslinking that was subsequently functionalized with the immobilized metal affinity (IMA) group, that included a spacing element, (1, 4 Butanediol Diglycidyl ether (1,4 BDE)), chelator (NTA (Nitrilotriacetic acid)), and metal (Nickel (Ni2+). The impact of chelating ligand and glutaraldehyde concentration on the swelling and bound metal content was determined. Additionally, Fourier-transform infrared spectroscopy (FTIR) was used to examine the functional groups for the control (without metal attached) and affinity (with metal attached) membranes. Ultimately, this study suggests that crosslinking membranes with a higher glutaraldehyde percentage yielded a higher content of bound nickel.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
