Abstract
Propolis, a bee product composed mainly of plant originated resins and bee wax, is one of the most popular traditional remedy for its beneficial effect on human health. However, the application of propolis on food is still limited due to its low water solubility. In this research, we treated lipase to remove bee wax from conventional propolis and consequently confirmed the safety of propolis in animal model. No adverse effect of enzyme treated PE was observed in the animal study. In the skin irritation test, neither treated lipase PE nor non‐treated lipase PE induced adverse skin reactions, such as erythema or edema. In skin sensitization, PE, Lipase OF and Lipozyme TL IM had a positive skin sensitization at the rate of 14%, 33% and 14%, respectively. Conclusively, skin safety of propolis can be enhanced by enzymatic lipid removal process.
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