Abstract
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most widespread and destructive fungal diseases of wheat worldwide. The cultivation and growth of resistant wheat varieties are the most economical, effective, and environmental friendly methods to control stripe rust. Therefore, it is necessary to use new resistance genes to breed resistant wheat varieties. A single dominant gene temporarily designated as YrM8664-3, from a wheat-Leymus mollis introgression line M8664-3 highly resistant to Chinese predominant Pst races, is a potentially valuable source of stripe rust resistance for breeding. Herein, based on previous YrM8664-3 chromosome location results (bin 4AL13-0.59-0.66 close to 4AL12-0.43-0.59) and expression change information of candidate genes and bioinformatics analysis, several candidate genes with significantly different expression changes were then selected and verified by virus-induced gene silencing (VIGS). Two of the candidate genes temporarily designated as TaFBN [containing plastid lipid-associated proteins (PAP)_fibrillin domain in its protein] and Ta_Pes_BRCT [containing Pescadillo and breast cancer tumour suppressor protein C-terminus (BRCT) domain in its protein], produced the most significant resistance changes in the wheat-Pst interaction system after silencing. These two genes were further verified by Agrobacterium-mediated wheat genetic transformation technology. According to the identification of disease resistance, the resistance function of the candidate gene TaFBN was further verified. Then, the expression of TaFBN under hormone treatment indicated that TaFBN may be related to the salicylic acid (SA) and abscisic acid (ABA) signaling pathways. Combined with the expression of TaFBN in response to environmental stress stimulation, it can be reasonably speculated that TaFBN plays an important role in the resistance of wheat to Pst and is involved in abiotic stress pathways.
Highlights
IntroductionWheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases that affects wheat production
Wheat (Triticum aestivum L.) is one of the most important food crops in the world
At 12 hpi, strong DAB staining appeared in the guard cells directly contacted by the substomatal vesicle, and there was a significant difference in the H2O2 staining area of the infestation sites between M8664-3 and Mingxian 169
Summary
Wheat stripe rust, caused by Pst, is one of the most important diseases that affects wheat production. It has the characteristics of rapid outbreaks that cause regional epidemics and subsequent extensive and severe harm to wheat crops, and it has been found in almost all major wheat-producing areas in the world. Since the 1950s, several nationwide stripe rust epidemics have been recorded in China, among which, a yield loss of 6, 3.2, 2.65, and 1.3 billion kg of wheat occurred in 1950, 1964, 1990, and 2002, respectively (Wan et al, 2004). In 2017, wheat stripe rust was once again epidemic throughout China, affecting an area of 5.56 million hm, which was the largest annual occurrence since 2002 (Huang et al, 2018). The most effective, economical, and environmental friendly method to control stripe rust is to breed and grow disease-resistant wheat varieties
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