Functional status of neutrophils at 2,4-dichlorophenoxyacetic acid amine salt intoxication

Abstract

Aim. To study the influence of 2,4-dichlorophenoxyacetic acid amine salt subacute intoxication on the functional status of neutrophils. Methods. 2,4-dichlorophenoxyacetic acid amine salt (42 mg/kg) was administered intragastrically during 28 days. The number of leukocytes, neutrophils and lymphocytes in peripheral blood, intensity of oxygen-dependent metabolism (spontaneous and induced tetrazolium test), absorbency, microbicidal activity of neutrophils in conditions of oxygen-dependent microbicidal factors functioning and sodium azide mediated blockade, the level of myeloperoxidase and cation proteins in neutrophils were registered at days 7th and 14th after the toxicant administration discontinuation. Results. Subacute 2,4-dichlorophenoxyacetic acid amine salt intoxication resulted in leucopenia due to neutropenia, accompanied by inhibition of oxygen-dependent neutrophils killing as the result of peroxidase-independent microbicidal mechanisms suppression (reduced synthesis of oxygen active forms). A reduced activity of oxygen-independent killing mechanisms correlating with a decrease of cation proteins levels was also registered. On the 14 day, leucopenia was replaced by leukocytosis due to elevated numbers of neutrophils and lymphocytes in the peripheral blood. At the same time, the activity of both oxidative peroxidase-dependent and oxygen-independent microbicidal mechanisms in neutrophils continued to decrease, accompanied by reduction of mieloperoxidase activity, neutrophil absorbency and cation proteins levels. Conclusion. Subacute intoxication with 2,4-dichlorophenoxyacetic acid amine salt leaded to quantitative and functional disorders in neutrophils that persisted by the 14 day of follow-up, indicating a need for further study of of 2,4-dichlorophenoxyacetic acid amine salt immunotoxic action to find the possible opportunities for its pharmacological correction.