Abstract
The cell membrane of cultured chick embryo cells is permeable to Ca 2+ after treatment with mannitol. Ca 2+ uptake by mannitol-treated cells can be attributed to the mitochondria. Ca 2+ O quotients and acceptor control ratios of such cells and isolated mitochondria are identical. Digitonin exerts two time-dependent effects on mannitol-treated cells: it increases the maximum extent of Ca 2+ uptake by 5 – 20 percent. It also partially uncouples respiration from Ca 2+ accumulation. It is suggested that mannitol treatment of cultured cells provides an easy way to study Ca 2+ uptake by mitochondria in situ .
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