Abstract
We have investigated the regulation by divalent cations Mg 2+, Ca 2+ and Mn 2+ of the functional activity of the human integrin VLA-1 expressed on neuroblastoma NB100 cells. VLA-1-mediated adhesion of NB100 cells to ligand collagen type I was supported by either mM concentrations of extracellular Mg 2+ or μM levels of Mn 2+. In contrast, Ca 2+ alone did not induce activation of VLA-1 but exerted a potent inhibitory effect on the Mg 2+-supported cell adhesion. We have also demonstrated that VLA-1 can be directly activated by the stimulatory monoclonal antibody TS2/16 specific for the integrin β1 subunit, resulting in effective adhesion of NB100 cells to type I collagen. This study has been possible by using a novel blocking VLA-α1 specific monoclonal antibody, 5E8D9.
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