Abstract
Secondary mixed leukocyte culture (MLC) responses of T lymphocytes effecting graft-versus-host (GVH) reactivity have been compared with the response patterns of T lymphocytes primed in vitro. GVH reactions were initiated in donor-recipient combinations selected to involve genetic differences at the entire H-2 complex or individual K/D and I regions. GVH-primed lymphocytes, whether derived from spleen, liver, or lymph nodes, exhibited unexpectedly specific proliferative reactivity against I region-encoded gene products. In combinations involving both K/D plus I region differences, cytotoxic T lymphocytes were generated against the K/D antigens. These response patterns contrasted to those exhibited by MLC-primed T cells. MLC-primed cells elicited proliferative responses against both K/D and I region antigens and revealed a high cross-reactivity among I region gene products of various haplotypes. The different response patterns for in vivo versus in vitro primed cells do not appear to be artifactual but a result of the priming environment.
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