Abstract
Barley (Hordeum vulgare) is an important cereal grain that is used in a range of products for animal and human consumption. Crop yield and seed quality has been optimized during decades by plant breeding programs supported by biotechnology and molecular biology techniques. The recently completed whole-genome sequencing of barley revealed approximately 26,100 open reading frames, which provides a foundation for detailed molecular studies of barley by functional genomics and proteomics approaches. Such studies will provide further insights into the mechanisms of, for example, drought and stress tolerance, micronutrient utilization, and photosynthesis in barley. In the present review we present the current state of proteomics research for investigations of barley chloroplasts, i.e., the organelle that contain the photosynthetic apparatus in the plant. We describe several different proteomics strategies and discuss their applications in characterization of the barley chloroplast as well as future perspectives for functional proteomics in barley research.
Highlights
Barley (Hordeum vulgare) is one of the earliest domesticated cereals and it is the fourth most important crop world-wide in terms of total dry production, only exceeded by maize, rice, and wheat
Barley is by nature diploid, has a low chromosome number (2n = 14) and a large genome size (5.1 Gb), is easy to cross-breed and is able to grow under various climatic conditions
The main methods used in proteomics are: (1) protein and peptide separation techniques; (2) mass spectrometry; (3) biological sequence databases and computational query tools
Summary
Barley (Hordeum vulgare) is one of the earliest domesticated cereals and it is the fourth most important crop world-wide in terms of total dry production, only exceeded by maize, rice, and wheat. The main methods used in proteomics are: (1) protein and peptide separation techniques; (2) mass spectrometry; (3) biological sequence databases and computational query tools (summarized in Boxes 1 and 2). We foresee an increased effort in barley proteomics using state-of-the-art mass spectrometry based strategies for qualitative and quantitative characterization of barley proteins, organelles and regulatory networks.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
