Abstract
Transient receptor potential ankyrin 1 (TRPA1) from tetrapod vertebrates except rodents are activated by high temperature with a relatively clear threshold. Our recent investigation suggested that a gradual heat activation without clear threshold might be a common feature for TRPA1 of fish. To approach which animal first acquires TRPA1 as a threshold detector instead of a gradual heat sensor, here, we focused on TRPA1 from axolotls (Ambystoma mexicanum). We isolated a full-length cDNA of axolotl transient receptor potential ankyrin 1 (axTRPA1) and studied the functional properties by two-electrode voltage clamp method using Xenopus oocytes. Allyl isothiocyanate, caffeine, methyl anthranilate and carvacrol activated axTRPA1 channels. The results indicated that axTRPA1 is heat activated with the average threshold of 39.7°C, suggesting that axTRPA1 already has acquired the functional property of land animals.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
