Abstract
New functional microspheres consisting of diethylene glycol dimethacrylate (2G) and a methacryloyl monomer containing four amino acids, l-asparagynyl- l-alanyl- l-asparagynyl l-proline methyl ester (MA-NANPOMe) were synthesized by radiation induced co-polymerization. These four amino acids were previously identified as a part of the immunodominant region of the circumsporozoite (CS) protein of Plasmodium falciparum. The use of this synthetic peptide as an antigen in immunoassay for malaria is promising: the microspheres exposed to the sera of malaria patients showed marked fluorescence, while the spheres contacted with sera of healthy donors showed no fluorescence.
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