Functional Oligosaccharide DFA III Forming Enzyme Produced by Microorganisms

Abstract

Inulin is a polysaccharide found in various plants (chicory, dahlia, and so on). A unique oligosaccharide DFA III (di-fructose di-anhydride III) is formed from inulin with a special microbial enzyme. This enzyme was named as inulin fructotransferase (DFA III-producing) [EC 4.2.2.18]. The oligosaccharide DFA III has sweetness half as sucrose. The DFA III also has a special quality that promotes the assimilation of minerals (Ca, Fe, and so on) from intestines. Therefore, the oligosaccharide DFA III has a unique potential for the prevention of osteoporosis and iron deficiency anaemia. The sales in the market of DFA III were started in 2004 in Japan. Now, we can purchase a product of supplement containing the DFA III and a mineral in a pharmacy and a convenience store in Japan. In this article, we summarize the research on the oligosaccharide DFA III forming enzyme produced by the microorganisms.