Abstract
The presence of δ5-3β-HSDH, 17β-HSDH, 11β-HSDH, G-6-PDH and DPNH-diaphorase activity has been demonstrated in the interrenal cells of two frogs, R. tigrina and R. cyanophlyctis. The substrate specificity of δ5-3β-HSDH and 17β-HSDH was tested by utilizing different specific hydroxysteroids. DL5-3β-HSDH, G-6-PDH and NADH-diaphorase activity was relatively more in the peripheral region of the interrenal tissue compared to the cells in the middle region, apparently indicating a zonation of the adrenocortical tissue. The presence of 11β-HSDH, G-6-PDH and NADH-diaphorase has been also observed in the renal tubules which indicates that the renal tubules might convert hydroxysteroids to ketosteroids during steroid excretion. The presence of two types of adrenal medullary cells showing positive iodate and chromate reactions was observed.
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