Functional morphology of cardiac stomach of Nile catfish (Clarias gariepinus): Histological, scanning, and ultrastructural studies.

Abstract

The present study was done on 20 adult specimens of Nile catfish (Clarias gariepinus) to demonstrate the morphological characteristics of the cardiac region of the stomach. The cardiac mucosa was characterized by a large number of well-defined long folds. The surface epithelial cells were simple columnar type covered with distinct microvilli and connected by desmosomes. Few PAS- and AB- positive goblet cells were found between the surface epithelium. In addition, many lymphocytes, macrophages, and blood capillaries were seen in the epithelial layer. The lamina propria was exclusively occupied by simple branched gastric (cardiac) glands that fill most of the thickness of the mucosa and open into gastric pits. The gastric glands were composed of numerous secretory tubules that were lined with one type of cells with a cytoplasm containing numerous electron-dense granules, well-developed rER, mitochondria, and a large number of free ribosomes. Moreover, macrophages were distributed in the lamina propria and submucosa. Telocytes were observed in the cardiac region for the first time around the glands, blood vessels, between the muscular layer, and in the serosa. A large number of mast cells could be identified in the submucosa around the blood vessels. The presence of many immune cells in the wall of the cardiac stomach suggests involvement in immune response in addition to its digestive function. RESEARCH HIGHLIGHTS: The study exposed many cell types in the wall of the cardiac stomach of Nile catfish including mast cells, lymphocytes, and neutrophils that suggests an involvement in the immune response. The current study is the first one to highlight the distribution of telocytes in the fish stomach.