Abstract

SummaryIn order to develop a male-sterile eggplant (Solanum melongena L.), cytoplasm substitution lines of eggplant were produced by continuous backcrossing using S. kurzii Brace & Prain as cytoplasm donor and eggplant as nucleus donor. Analyses of chloroplast DNA and mitochondrial DNA confirmed that all backcross progenies had the cytoplasm from S. kurzii. Anthers in some BC1 and BC2 plants, and in most BC3 plants, were of the indehiscent-type (i.e., pollen non-release type). Pollen fertility remained low in all backcross progenies. The number of seeds per fruit in the backcross progenies increased in successive generations. Functional male-sterility of the anther- indehiscent-type was shown to be attributed to incompatibility between the cytoplasm of S. kurzii and nuclear genes of S. melongena. The present male-sterile lines are potentially valuable to reduce the cost of developing hybrid cultivars and to produce seedless eggplant fruit.

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