Functional magnetic nanoparticle-based affinity probe for MALDI mass spectrometric detection of ricin B.

Abstract

Theuse of lactosylated Fe3O4 magnetic nanoparticles (MNP@LAC) has been exploredas affinity probes against ricin B based on galactose-ricin B binding interactions. Lactose was bound onto the surface of aminated MNPs through the Maillard reaction. The enrichment of ricin B took ~1h by incubating MNP@LAC with samples under shaking at room temperature, followed by magnetic isolation. The resultant MNP@LAC-ricin B conjugates were characterized by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The limit of detection toward ricin B was ~3nM by using the developed method. It was possibleto detect the peptides derived from the tryptic digest of trace ricin B (~0.39nM) enriched by the MNP@LAC probes followed by tryptic digestion and MALDI-MS analysis. The feasibility of using the developed method for detection of ricin B from complex white corn starch samples spiked with trace ricin B was demonstrated.