Abstract
Stable clonal cell line of hCEC, HEN7, has been generated from human fetal telencephalon using a retroviral vector encoding human telomerase reverse transcriptase (hTERT). HEN7 cells were transplanted intravenously in rat brain with photochemically induced focal cerebral ischemia, and the clinical effects for infarct size, edema volume, and clinical outcome were evaluated. FACS analysis showed HEN7 cells express the characteristics of cerebral endothelial cells. Matrigel assay showed HEN7 cells are capable of good tube formation. HEN7 showed positive immunoreactivity for vascular, stem cell, and tight junction proteins. HEN7 transplanted group showed reduced infarct lesion as identified by bioluminescence and X‐gal staining located in the infracted lesion border area. HEN7 transplanted group showed markedly reduced edema volume, MMP‐9 expression, and Caveolin‐1 expression. HEN7 transplanted group showed earlier recovery from the neurological deficit.Intravenously transplanted hCECs selectively migrated and integrated into cerebral ischemic lesion area and accelerate neurological functional recovery. This new hCEC‐based cellular therapy is applicable for clinical trial in ischemic stroke patients.
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