Abstract
Bacillus siamensis strain B28 was previously isolated from traditional Korean fermented kimchi and inhibited expression of the microphthalmia-associated transcription factor and β-catenin in human embryonic kidney 293 cells. Here, we determined the complete genome sequence of strain B28 and compared it with other strains to elucidate its potential probiotic properties. Strain B28 does not contain antibiotic resistance-, hemolysin- or enterotoxin-encoding genes. The genome includes genes related to survival in extreme conditions, adhesion in the gut, and synthesis of the bacteriocin. Considering the potential for enhancement of human health, the strain B28 genome encodes genes related to production of eight essential amino acids, γ-aminobutyric acid, branched-chain fatty acids, γ-glutamyltransferase, and subtilisin. There are genes for the synthesis of uracil, lipoteichoic acid, glutathione, and several reactive oxygen species-scavenging enzymes. Experimentally, strain B28 exhibited sensitivity to eight antibiotics and antibacterial activity against seven foodborne pathogens. B. siamensis B28 is a safe strain with potential for development as a probiotic.
Highlights
The Bacillus genus comprises spore-forming bacteria found in various niches, such as oil, agricultural crops, fermented foods, and the human gastrointestinal tract [1,2,3,4,5,6].Bacillus sp. can be detected in kimchi using culture-dependent and -independent methods [7,8]
Several B. polyfermenticus strains have been reclassified: B. polyfermenticus GF423 was renamed B. velezensis variant polyfermenticus GF423 based on genome sequencing [21], and B. polyfermenticus KMU01 was reclassified as B. velezensis
Strain B28 was isolated from kimchi and identified as B. polyfermenticus in a previous investigation [19]
Summary
The Bacillus genus comprises spore-forming bacteria found in various niches, such as oil, agricultural crops, fermented foods, and the human gastrointestinal tract [1,2,3,4,5,6]. Bacillus spp. play important roles in the enhancement of the sensory properties of fermented foods via their amylolytic, lipolytic, and proteolytic activities [9,10,11,12,13]. Some Bacillus strains exhibit antibacterial activity against food pathogenic bacteria [15,16], anticancer effects on human colon cancer cells [17], and probiotic properties in humans and animals [18]. A B. polyfermenticus strain named B28 isolated from kimchi inhibited the expression of microphthalmia-associated transcription factor and β-catenin by human embryonic kidney 293 cells [19]. Several B. polyfermenticus strains have been reclassified: B. polyfermenticus GF423 was renamed B. velezensis variant polyfermenticus GF423 based on genome sequencing [21], and B. polyfermenticus KMU01 was reclassified as B. velezensis. We undertook comparative genomic analysis to understand the functionality and safety of strain B28 and to assess its probiotic properties
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