Abstract
Antibiogram profile of 1590 clinical bacterial isolates based on thirteen different antimicrobial compounds showed that 1.6% of the bacterial isolates are multidrug resistant. Distribution pattern based on 16S rRNA sequence analysis showed that Pseudomonas aeruginosa constituted the largest group (83.6%) followed by Burkholderia pseudomallei sp. A191 (5.17%), Staphylococcus sp. A261 (3.45%). Among the various antibiotics used, colistin appeared to be the most effective against the Gram negative bacteria. Burkholderia pseudomallei sp. A191 and Pseudomonas aeruginosa sp. A111 showed resistance to 1500 μg/ml and 750 μg/ml of colistin respectively which constitutes 7.7% of the bacterial population. A functional genomics strategy was employed to discover the molecular support for colistin resistance in Burkholderia pseudomallei sp. A191. A pUC plasmid-based genomic expression library was constructed with an estimated library size of 2.1 × 107bp. Five colistin resistant clones were obtained after functional screening of the library. Analysis of DNA sequence of five colistin resistant clones showed homology to two component regularity systems (TCRS) encoding for a histidine kinase (mrgS) and its regulatory component (mrgR). Cross complementation assay showed that mutations in mrgS were sufficient enough to confer colistin resistant phenotype in a sensitive strain.
Highlights
Outbreaks of infections with multi drug resistant (MDR) strains in intensive care unit settings have been reported in several countries across the world
We investigated the mechanism of colistin resistance by functional genome screening in Burkholderia pseudomallei
A total of 1590 bacterial isolates were collected from clinical samples isolated from patients admitted to various wards of the hospital
Summary
Mohit Kumar[1], Ashutosh Gupta1,*, Rajesh Kumar Sahoo3,*, Jayanti Jena2,*, Nagen Kumar Debata2 & Enketeswara Subudhi[3]. Hospitals are crowded with debilitated patients who are administered with heavy doses of broad-spectrum antibiotics often without diagnosing specific infecting organism and without following any proper guidelines leading to an ineffective treatment. This brings in a threat to the patient survival and to curb spread of infection[3]. Outbreaks of infections with multi drug resistant (MDR) strains in intensive care unit settings have been reported in several countries across the world The treatment of these infections has become difficult due to growing prevalence of pan drug resistance (PDR). We investigated the mechanism of colistin resistance by functional genome screening in Burkholderia pseudomallei
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