Abstract
Aim. To study the egg quality traits of Poltava Clay chicken line 14 and Rhode-Island Red chicken line 38 with different genotypes of the prolactin gene (PRL), growth hormone gene (GH), growth hormone receptor gene (GHR), insulin-like growth factor I gene (IGF-I) and Mx gene (Mx). Methods. The study was conducted using the method of polymerase chain reaction and restriction fragment length polymorphism analysis (PCR-RFLP). Results. We found signifi cant differences in line 14 for egg quality between prolactin, growth hormone, growth hormone receptor and Mx loci. Homozygous individuals CC and TT by prolactin locus prevailed over heterozygotes CT for egg weight on the 30th week of life. As for the growth hormone gene, the maximum differences for egg weight were revealed when comparing BC heterozygotes with CC homozygotes. As for the growth hormone receptor gene, signifi cant prevalence (p < 0.05) of individuals with the B0 genotype over A0 by parameters of egg yolk weight was noted at the age of 52 weeks. Signifi cant differences (p < 0.05) in eggshell thickness were determined for genotypes AG and GG by Mx gene in week 52. There were signifi cant differences (p < 0.05) in egg quality traits for prolactin and Mx gene for chickens of line 38. TT homozygotes by prolactin locus are characterized by the prevalence of values (p < 0.05) for the egg, yolk and shell weight. In case of Mx gene polymorphism, the heterozygous individuals were characterized by signifi cantly higher values (p < 0.05) of egg and albumen weight on the 30th week of life. There were no signifi cant differences in both experimental chicken lines for other egg quality traits between individuals with different genotypes. Conclusions. The data obtained are recommended for the use in breeding programs for Poltava Clay chicken line 14 and Rhode-Island Red chicken line 38 with the aim of obtaining microlines with the different genotypes for PRL, GH, GHR and Mx loci.
Highlights
The aim of this study was to investigate the relation between egg quality traits of Poltava Clay chicken line 14 and Rhode-Island Red chicken line 38 and different genotypes for the prolactin gene (PRL), growth hormone gene (GH), growth hormone receptor gene (GHR), insulin-like growth factor I gene (IGF-I) and Mx gene (Mx)
We found different alleles and genotypes in each experimental population by all the other loci
We have analyzed the relation of egg quality traits of Poltava Clay chicken line 14 and Rhode-Island Red chicken line 38 with different genotypes of the prolactin loci, growth hormone (AluI and MspI-polymorphism in intron 4), growth hormone receptor (HindIII-polymorphism in intron 2), insulin-like growth hormone factor I gene (HinfI-polymorphism in promoter fragment) and Mx gene
Summary
The aim of this study was to investigate the relation between egg quality traits of Poltava Clay chicken line 14 and Rhode-Island Red chicken line 38 and different genotypes for the prolactin gene (PRL), growth hormone gene (GH), growth hormone receptor gene (GHR), insulin-like growth factor I gene (IGF-I) and Mx gene (Mx)
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