Abstract
We recently succeeded in resuscitating an extracted rat heart following 24–48 hours of preservation in a high-pressure gaseous mixture of carbon monoxide (CO) and oxygen (O2). This study aimed to examine the function of rat hearts transplanted after being preserved in the high-pressure CO and O2 gas mixture. The hearts of donor rats were preserved in a chamber filled with CO and O2 under high pressure for 24 h (CO24h) or 48 h at 4 °C. For the positive control (PC) group, hearts immediately extracted from donor rats were used for transplantation. The preserved hearts were transplanted into recipient rats by heterotopic cervical heart transplantation. CO toxicity does not affect the grafts or the recipients. Light microscopy and [18F]-fluorodeoxyglucose positron emission tomography revealed that there were no significant differences in the size of the myocardial infarction or apoptosis of myocardial cells in post-transplant hearts between the PC and CO24h groups. Furthermore, at 100 days after the transplantation, the heart rate, weight and histological staining of the post-transplanted hearts did not differ significantly between the PC and CO24h groups. These results indicate that the function of rat hearts is well preserved after 24 hours of high-pressure preservation in a CO and O2 gas mixture. Therefore, high-pressure preservation in a gas mixture can be a useful method for organ preservation.
Highlights
Is used in very limited conditions and after heart surgery, and extended high concentrations of inhaled O2 are usually avoided[15]
Improvement of the University of Wisconsin (UW) solution[18], a two-layer method using UW19, and super cooling with UW20 have all been attempted. These attempts have resulted in the stock solution having organ- and/or cell-protective effects because immersion in a stock solution is generally considered important to organ preservation[10,11]
The post-transplanted hearts in the CO24h and CO48h groups could survive for 100 days after transplantation. These results indicate that a high-pressure preservation in a mixture of CO and O2 gases is useful for maintaining the quality of the long-term preserved donor heart
Summary
Is used in very limited conditions and after heart surgery, and extended high concentrations of inhaled O2 are usually avoided[15]. A condition of high-pressure CO (CO = 4000 hPa +PHe = 3000 hPa) could not preserve the rat hearts for 48 hours (data not shown). It seems that a combination of CO and O2 is needed for organ preservation. We developed a high-pressure preservation method using a mixture of CO and oxygen (O2) gases and succeeded in resuscitating extracted rat hearts following 24–48 hours of preservation under a condition of 7000 hPa {Partial Pressure (P) CO = 4000 hPa +PO2 = 3000 hPa}12. This study aimed to examine the function of rat hearts transplanted after high-pressure preservation in a mixture of CO and O2 gases
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