Functional condition of microcirculation in the zone of intestinal anastomosis during postoperative period upon stimulation of its regeneration (experimental study)

Abstract

Objectives: to conduct a comparative assessment of the functional condition of microcirculation of the digestive anastomosis zone in the early and delayed postoperative period upon local usage of autogenic growth factors (PRP). The experiment was performed on 30 mature laboratory rabbits. All animals underwent the imposition of two digestive "end-to-end" intestinal anastomosis: the control one at a distance of 15 cm from the ileocecal angle and the experimental one retreating 30 cm from the imposed anastomosis. The experimental anastomosis differed from the control one by using a method of local stimulation of its regeneration via injecting of platelet-riched-plasm (PRP). A comparative assessment of the functional state of the microcirculation of the intestinal anastomosis zone was carried out on the 3rd, 7th, 14th days in the postoperative period using the method of laser doppler flowmetry (LDF). The obtained data determined the ability of the PRP method to improve the conditions of the regenerative process in the intestinal anastomosis zone by increasing the rate of basal blood flow and tissue perfusion, the rates of which have maximum values in the early postoperative period (3 day).