Abstract
Neutrophils of cows injected with endotoxin were evaluated for their functional competence and monoclonal antibody binding in relation to morphological maturity. Six clinically healthy lactating Holstein cows were each injected intravenously with 100 μg Escherichia coli endotoxin. Blood was collected at 0, 6, 30, 54, 78 and 150 hours postinjection for total and differential leucocyte counts and to isolate neutrophils for functional assays. Marked leucopenia, neutropenia with left shift to band neutrophils, lymphopenia and monocytopenia were evident at 6 h postinjection. Neutrophil and lymphocyte counts normalised by 30 hours, while band neutrophils remained elevated until 54 hours postinjection.
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