Abstract
An Arabidopsis thaliana gene, At1g56550, was expressed in Pichia pastoris and the recombinant protein was shown to catalyse transfer of d-xylose from UDP-α- d-xylose onto methyl α- l-fucoside. The product formed was shown by 1D and 2D 1H NMR spectroscopy to be Me α- d-Xyl-(1,3)-α- l-Fuc, which is identical to the proposed target structure in the A-chain of rhamnogalacturonan II. Chemically synthesized methyl l-fucosides derivatized by methyl groups on either the 2-, 3- or 4 position were tested as acceptor substrates but only methyl 4- O-methyl-α- l-fucopyranoside acted as an acceptor, although to a lesser extent than methyl α- l-fucoside. At1g56550 is suggested to encode a rhamnogalacturonan II specific xylosyltransferase.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
