Functional assessment and tissue response of short- and long-term absorbable surgical meshes

Abstract

Within the last few years meshes have become essential for the temporary closure of the abdominal cavity to avoid the development of an intra-abdominal compartment syndrome. The interposition of a mesh as an inlay reduces the intra-abdominal pressure and improves markedly the blood circulation, particularly for the intestines and kidneys. Whereas non-absorbable meshes usually tend to produce fistulas in direct contact to the bowels, the interposition of short-term absorbable meshes result in large incisional hernias in almost all cases. In the following study we investigated the functional and histological consequences of a short-term absorbable mesh (polyglactin 910, Vicryl ®, loss of 50% of its mechanical stability within 3 weeks) and a long-term absorbable mesh (polylactide, LTS, preserved >50% of its mechanical stability for over 1 year). The mesh-modifications were both tested with the aid of three-dimensional stereography, tensiometry, light- (LM) and transmission electron microscopy (TEM) as well as morphometry after implantation intervals of 3, 7, 14, 21, 45, 90, 135 and 180 days in a standardised rat model. The PG-mesh initially revealed a pronounced inflammatory reaction and a significantly increased formation of connective tissue. The extensive arrangement of connective tissue in the interface mesh/recipient tissues correlated to an increased stiffness of the abdominal wall compared to the sham-group. However, a loss of mechanical stability and an increase of elasticity could be detected after 3 weeks of implantation which may be explained by the rapid absorption of the mesh material. In contrast to PG, the LTS-mesh indicated a decreased but persisting inflammatory reaction in the interface mesh-fibres/recipient tissues and a significantly reduced induction of connective tissue. Although, the formation of scar-tissue was diminished compared to PG the LTS-mesh preserved its mechanical stability after 180 days. The results indicate that the frequent development of incisional hernias with short-term absorbable meshes (PG) might be due to the decreased mechanical stability and dilatation of the newly formed connective tissue after 2–3 weeks. Moreover, extensive scar tissue development may promote adhesion formation. The implantation of the long-term absorbable LTS-mesh seems to be favourable with respect to its long-term mechanical stability and the decreased connective tissue formation.