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Abstract
Two molecular forms of GnRH were identified in the African catfish, Clarias gariepinus: catfish gonadotropin-releasing hormone (cfGnRH) and chicken gonadotropin-releasing hormone (cGnRH-II). They differ in gonadotropine hormone (GTH)-release activity, cGnRH-II being about a hundred times as potent as cfGnRH. This difference corrcsponds to different GnRH receptor binding affinities. cDNAs encoding both GnRH preprohormones were cloned and specific antibodies were raised against the GnRHs. By immunocytochemistry and in situ hybridization cGnRH-II was localized in the midbrain tegmentum, cfGnRH in the rostro-ventral forebrain.
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