Functional and metabolic neutrophils activity in patients with acute intestinal infections and the influence of selimakcid

Abstract

Aim. To explore the functional activity of neutrophils in healthy individuals and patients with acute intestinal infections and the effect of selimakcid on it in vitro. Methods. 48 patients with acute intestinal infections aged 20 to 60 years were examined. The control group consisted of 10 healthy people of the same age. Functional and metabolic activity of neutrophils was studied by a nitro blue tetrazolium chloride test and by «BURSTTEST» (Neutrophil Oxidative Burst Test). Results. Examination of the functional and metabolic activity of neutrophils in patients with acute intestinal infections revealed increased results of spontaneous nitro blue tetrazolium chloride test in acute period of the disease. The same changes were discovered while studying the Serratia marcescens stimulated cell activity in patients with dysentery. However, in patients with salmonellosis neutrophils activity in induced nitro blue tetrazolium chloride test did not differ from that of healthy controls. The number of neutrophils synthesizing reactive oxidants recorded by «BURSTTEST» in patients corresponded to the indicators of the induced nitro blue tetrazolium chloride test and did not differ in patients with different nosologies. Studying the effect of selimakcid on neutrophils functional activity in healthy controls and patients with acute intestinal infections by nitro blue tetrazolium chloride test and «BURSTTEST» showed that pre-incubation of neutrophils with selimakcid intensified the response to Serratia marcescens. The ability of the drug to modulate the neutrophils activity in patients with acute intestinal infections was higher compared to healthy individuals. Conclusion. Increased functional activity of neutrophils associated with reduced reserve capacity was revealed in patients with salmonellosis and shigellosis of moderate severity. Selimakcid showed immunomodulatory effect, increasing the functional and metabolic potential of neutrophils in patients with acute intestinal infections in vitro.