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Abstract
Sugar content is related to fruit sweetness, and the complex mechanisms underlying fruit sugar accumulation still remain elusive. Here, we report a peach PpTST1 gene encoding tonoplast sugar transporter that is located in the quantitative trait loci (QTL) interval on Chr5 controlling fruit sucrose content. One derived Cleaved Amplified Polymorphic Sequence (dCAPS) marker was developed based on a nonsynonymous G/T variant in the third exon of PpTST1. Genotyping of peach cultivars with the dCAPS marker revealed a significant difference in fruit sugar content among genotypes. PpTST1 is located in the tonoplast, and substitution of glutamine by histidine caused by the G/T variation has no impact on subcellular location. The expression profile of PpTST1 exhibited a consistency with the sugar accumulation pattern, and its transient silencing significantly inhibited sugar accumulation in peach fruits. All of these results demonstrated the role of PpTST1 in regulating sugar accumulation in peach fruit. In addition, cis-elements for binding of MYB and WRKY transcript factors were found in the promoter sequence of PpTST1, suggesting a gene regulatory network of fruit sugar accumulation. Our results are not only helpful for understanding the mechanisms underlying fruit sugar accumulation, but will also be useful for the genetic improvement of fruit sweetness in peach breeding programs.
Highlights
The peach (Prunus persica (L.) Batsch) is considered an important economical fruit crop worldwide
To investigate whether PpTST1 had a potential role in the regulation of sugar accumulation in peach fruit, its expression profile along with sugar accumulation were examined in peach fruit
The expression profile of PpTST1 displayed a consistency with the sugar accumulation pattern in peach fruits, suggesting a potential role of PpTST1 in the regulation of fruit sugar accumulation
Summary
The peach (Prunus persica (L.) Batsch) is considered an important economical fruit crop worldwide. Flavor is one of the key factors affecting fruit quality, and it largely depends on soluble sugar and organic acid contents [1]. Sucrose accounts for over 54% of total soluble sugars, which are mainly stored in the vacuole, occupying up to 90% of the total cell volume [3]. The mechanisms underlying sugar accumulation in peach fruit remain largely unknown. Tonoplast sugar transporters (TSTs) are capable of loading soluble sugars into the vacuole. The first TST was identified in Arabidopsis; it was designated tonoplast monosaccharide transporter (TMT) as its lethal mutation caused a significant decrease in monosaccharide accumulation [7]. An increasing number of studies are showing that TMTs function as proton-coupled antiporters that are able to transport sucrose into the vacuole [8,9]. TMTs were renamed as TSTs in a recent study [9]
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