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Abstract
Dnop5 is a member of the conserved nop5/sik1 gene family, which encodecomponents of small nucleolar ribonucleoprotein(snoRNP) complexes. To study thefunction of DNop5, we generated the polyclonal antibody and determined its expressionpattern. It is highly expressed in different periods of the Drosophila development. We usedheritable RNA interference (RNAi) in combination with the yeast GAL4/UAS binarysystem to knock down the DNop5 protein. It resulted in lethality and dramatic somaticanomalies in RNAi mutant fly, in which the DNop5 protein is reduced efficiently. Northernblotting showed that the processing of 18S rRNA was disrupted in DNop5 knock down fly,but 28S rRNA is normal. These results suggest that DNop5 is essential for the Drosophilagrowth and function in the execution of early pre-rRNA processing steps that lead toformation of 18S rRNA.
Highlights
In eukaryotes, rRNA genes are transcribed as precursor RNAs in a specialized nuclear compartment, the nucleolus, and undergo extensive processing that includes covalent modification of highly conserved regions and a series of endo-nucleolytic and exonucleolytic cleavages
Dnop5 is a member of the conserved nop5/sik1 gene family, which encode components of small nucleolar ribonucleoprotein(snoRNP) complexes
These results suggest that DNop5 is essential for the Drosophila growth and function in the execution of early pre-rRNA processing steps that lead to formation of 18S rRNA
Summary
RRNA genes are transcribed as precursor RNAs (pre-rRNAs) in a specialized nuclear compartment, the nucleolus, and undergo extensive processing that includes covalent modification of highly conserved regions and a series of endo-nucleolytic and exonucleolytic cleavages. Nop5/sik gene encode components of small nucleolar ribonucleoprotein (snoRNP) complexes, snoRNPs mainly catalyze the modification of rRNA. It is identified that human Nop5/Nop is component of the box C/D class of snoRNPs. In yeast, Nop5p functions with Nop1p in the execution of early pre-rRNA processing steps that lead to formation of 18S rRNA [3]. Nop5p functions with Nop1p in the execution of early pre-rRNA processing steps that lead to formation of 18S rRNA [3] It is not clear whether this is the case in Drosophila. A novel gene in Drosophila, is the member of the conserved nop5/sik gene family. To characterized the expression profile of DNop, we prepared the polyclonal antibodies against DNop and found that it is expressed dynamically and stably during Drosophila development. We present some evidences that DNop is associated with fly development and required for 18S rRNA synthesis
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