Abstract
Arbuscular mycorrhizal symbiosis is a mutualistic endosymbiosis widely distributed in the plant kingdom which has a significant impact on plant growth and health. Agrobacterium rhizogenes-mediated root transformation and composite plant generation have been described as a rapid method to assess gene functions in roots without the need for stable transformation plant production. We describe an optimized protocol for composite tomato plant obtaining achieved through A. rhizogenes-mediated transformation, and we also highlight key differences with other protocols that should be taken into account to adjust this method to the transformation of other plant species. This protocol has been adopted as a useful tool for localizing the promoter expression of genes putatively associated with mycorrhization or for functional analyses in mycorrhizal studies by reverse genetics.
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