Abstract
Methylomonas sp. DH-1, newly isolated from the activated sludge of a brewery plant, has been used as a promising biocatalytic platform for the conversion of methane to value-added chemicals. Methylomonas sp. DH-1 can efficiently convert methane and propane into methanol and acetone with a specific productivity of 4.31 and 0.14 mmol/g cell/h, the highest values ever reported, respectively. Here, we present the complete genome sequence of Methylomonas sp. DH-1 which consists of a 4.86 Mb chromosome and a 278 kb plasmid. The existence of a set of genes related to one-carbon metabolism and various secondary metabolite biosynthetic pathways including carotenoid pathways were identified. Interestingly, Methylomonas sp. DH-1 possesses not only the genes of the ribulose monophosphate cycle for type I methanotrophs but also the genes of the serine cycle for type II. Methylomonas sp. DH-1 accumulated 80 mM succinate from methane under aerobic conditions, because DH-1 has 2-oxoglutarate dehydrogenase activity and the ability to operate the full TCA cycle. Availability of the complete genome sequence of Methylomonas sp. DH-1 enables further investigations on the metabolic engineering of this strain for the production of value-added chemicals from methane.
Highlights
Methane is the principal component of natural/shale gas and biogas, and recently, has attracted much attention as a chemical feedstock [1]
Eight contigs from Fw12E-YT were aligned consecutively with this sequence and complete gene sequences were found from strains LM6, R-49807, and R-45378, while no homologous sequence could be found from other genomes, which suggests that this is a common characteristic of the Methylomonas koyamae species that could increase bacterial fitness under specific environmental niches [11]
DH-1 consisting of a 4.86 Mb chromosome and a 278 kb plasmid
Summary
Methane is the principal component of natural/shale gas and biogas, and recently, has attracted much attention as a chemical feedstock [1]. In an effort to develop a biocatalytic platform strain, we have isolated a type I methanotroph, Methylomonas sp. DH-1 was reported as a highly efficient biocatalyst for the bioconversion of methane to methanol, which can be directly used as alternative fuels, antifreeze and as a precursor to other compounds, with a specific productivity of 4.31 mmol/g cell/h [4]. The accumulation of acetone in the absence of chemical inhibitors is advantageous. The accumulation of acetone in the absence of chemical inhibitors is advantageous for for biocatalytic. DH-1 can can produce produce yellow-to-red pigments which are expected to be numerous carotenoids (unpublished report). Yellow-to-red pigments which are expected to be numerous carotenoids (unpublished report). DH-1 as the first step for the development of a methanotrophic platform strain.
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