Abstract
BackgroundRenal cell carcinomas (RCCs) overexpress fatty acid binding protein 7 (FABP7). We chose to study the TUHR14TKB cell line, because it expresses higher levels of FABP7 than other cell lines derived from renal carcinomas (OS-RC-2, 786-O, 769-P, Caki-1, and ACHN).MethodsFABP7 expression was detected using western blotting and real-time PCR. Cell proliferation was determined using an MTS assay and by directly by counting cells. The cell cycle was assayed using flow cytometry. Cell migration was assayed using wound-healing assays. An FABP7 expression vector was used to transfect RCC cell lines.ResultsThe levels of FABP7 expressed by TUHR14TKB cells and their doubling times decreased during passage. High-passage TUHR14TKB cells comprised fewer G0/G1-phase and more S-phase cells than low-passage cells. Cell proliferation differed among subclones isolated from cultures of low-passage TUHR14TKB cells. The proliferation of TUHR14TKB cells decreased when FABP7 was overexpressed, and the cell migration property of TUHR14TKB cells were decreased when FABP7 was overexpressed. High concentrations of docosatetraenoic acid and eicosapentaenoic acid accumulated in TUHR14TKB cells that overexpressed FABP7, and docosatetraenoic acid enhanced cell proliferation.ConclusionsThe TUHR14TKB cell line represents a heterogeneous population that does not express FABP7 when it rapidly proliferates. The differences in FABP7 function between RCC cell lines suggests that FABP7 affects cell proliferation depending on cell phenotype.
Highlights
Renal cell carcinomas (RCCs) overexpress fatty acid binding protein 7 (FABP7)
The levels of FABP7 expressed by TUHR14TKB cells decreased by approximately fourfold between two cell passages (Fig. 1b)
In the presence of 10% fetal bovine serum (FBS), the doubling time of TUHR14TKB cells that overexpressed FABP7 was significantly longer than that of cells transfected with the control vector (Fig. 4a and b)
Summary
Renal cell carcinomas (RCCs) overexpress fatty acid binding protein 7 (FABP7). In 2008, approximately 271,000 new cases were diagnosed, and 116,000 patients died from this disease [1]. These rates are approximately twice as high in men as in women [1]. Renal cell carcinomas (RCCs) represent 91.6% of kidney cancers [2]. The identification of molecular markers in body fluids, which can be used for screening, diagnosis, follow-up, and monitoring drugbased therapy of patients with RCC, is one of the most important challenges of cancer research [3]. In a search for candidate markers of RCC, we identified the gene (FABP7) encoding fatty acid binding protein 7 [4]
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