Functional Analysis of a type-2C Protein Phosphatase (AtPP2C52) in <i>Arabidopsis thaliana</i>

Abstract

AtPP2C52 is a plasma membrane type-2C protein phosphatase. In this study, AtPP2C52 promoter-GUS analysis revealed that AtPP2C52 gene was found in a broad expression spectrum with a higher level in the vascular and meristem. AtPP2C52 can interact with multiple proteins, including a proteasome maturation factor, UMP1, and a cysteine proteinase, RD21a, as well as the heterotrimeric G proteins β subunit, AGB1. By mutational analysis of AtPP2C52, it was identified that some residues were essential for AtPP2C52 to bind AGB1, UMP1 and RD21a, suggesting that these proteins should be potential substrates of AtPP2C52.