Abstract
Regardless of whether a leaf is simple or compound, the mechanism underlying its development will give rise to a full comprehension of plant morphogenesis. The role of Argonaute1 (AGO1) in the development of simple leaves has been established, but its role in the development of compound leaves remains to be characterized. In this paper, a virus-induced gene silencing (VIGS) strategy was used to dramatically down-regulate the expression of AGO1 ortholog in tomatoes, a model plant for research into compound leaves. AGO1-silenced tomato compound leaves exhibited morphological defects of leaf adaxial-abaxial and trichome development. Analysis of global gene expression profiles indicated that the silencing of AGO1 in tomato compound leaf caused significant changes in the expression of several critical genes, including Auxin Response Factor 4 (ARF4) and Non-expressor of PR5 (NPR5), which were involved in adaxial-abaxial formation and IAA15 that was found to contribute to growth of trichomes as well as Gibberellic Acid Insensitive (GAI) which participated in hormone regulation. Collectively, these results shed light on the complicated mechanism by which AGO1 regulates compound leaf development.
Highlights
Leaf development is divided into three continuous main stages: initiation, primary morphogenesis, and secondary morphogenesis
When the virus infects plants and spreads systemically throughout them, the endogenous gene transcripts, which are homologous to the insert fragment in the virus vector, are degraded by post-transcriptional gene silencing (PTGS) [34]
Mi et al supposed that virus-induced gene silencing (VIGS) was compromised in the ago1 mutant, with greatly reduced photo bleaching when injected with tobacco rattle virus (TRV)-PDS [33]
Summary
Leaf development is divided into three continuous main stages: initiation, primary morphogenesis, and secondary morphogenesis. The blade is initiated and forms (primary morphogenesis stage). The leaf area grows substantially, forming a leaf (secondary morphogenesis stage) [1]. Leaves show great diversity in their shape and size, they are normally divided into two major morphogenetic groups: simple and compound. Simple leaves are each a single unit consisting of a petiole and a blade (lamina). Simple leaf development has been well characterized at the molecular level, and many genes and pathways have been found to be involved [3]. The morphogenesis stage is longer in compound leaves than in simple leaves, allowing for the initiation of leaflets [1]. The blades of compound leaves are divided into several units called
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