Abstract
BackgroundIn the poultry and livestock industries, precise genetic information is crucial for improving economic traits. Thus, functional genomic studies help to generate faster, healthier, and more efficient animal production. Chicken myoblast cells, which are required for muscle development and regeneration, are particularly important because chicken growth is closely related to muscle mass.ResultsIn this study, we induced expression of microRNA-146b-5p mediated by the piggyBac transposon system in primary chicken myoblast (pCM) cells. Subsequently, we analyzed and compared the proliferation and differentiation capacity and also examined the expression of related genes in regular pCM (rpCM) cells and pCM cells overexpressing miRNA-146b-5p (pCM-146b OE cells). pCM-146b OE cells showed increased proliferation and upregulated gene expression related to cell proliferation. In addition, next-generation sequencing analyses were performed to compare global gene expression patterns between rpCM cells and pCM-146b OE cells. We found that the higher proliferation in pCM-146b OE cells was the result of upregulation of gene sets related to the cell cycle. Moreover, miRNA-146b-5p overexpression had inhibitory effects on myotube differentiation in pCM cells.ConclusionsCollectively these results demonstrate that miR-146b-5p is closely related to the proliferation and differentiation of chicken myogenic cells as a modulator of post-transcription.
Highlights
In the poultry and livestock industries, precise genetic information is crucial for improving economic traits
Results miR-146b-5p overexpression in primary chicken myoblast (pCM) cells Based on our previous study using a miRNA expression system [24], we designed and constructed a piggyBac transposon-mediated miR-146b-5p overexpression vector
Expression of miR-146b-5p was significantly upregulated in pCM-146b miR-146b-5p overexpressing pCM (OE) cells compared to regular pCM (rpCM) cells (Fig. 1c)
Summary
In the poultry and livestock industries, precise genetic information is crucial for improving economic traits. Understanding the genes or genetic markers involved in biological functions and regulatory pathways can help to improve economically important traits in the poultry industry [1,2,3]. Functional genomic studies are powerful and effective for investigating the modulatory mechanisms between cell proliferation and differentiation, in particular in skeletal muscles [4,5,6,7]. There are numerous reports of miRNAs controlling developmental and cellular processes such as cell proliferation, differentiation, and tissue specification [13, 14]. MiR-146b-5p is a key regulator of muscle regeneration and myoblast differentiation in mice [20]. MiR-146b-3p controls myoblast proliferation and differentiation in chicken [21]
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