Abstract
Objective To investigate the function of peroxisome proliferator-activated receptor β/δ (PPARβ/δ) expression in non-small cell lung cancer (NSCLC).Methods Reverse transcription-poly-merase chain reaction (RT-PCR) and PPARβ/δ luciferase activity experiment to detect PPARβ/δ,PPARγ,phospholipase A2 (PLA2),cytochrome C oxidase 2 (Cox-2),prostaglandin E synthase (PGES),prostacyclin synthase (PGIS) expression level in NSCLC cell lines,NSCLC tissue and normal tissue sam-ple from NSCLC patients.The effect of promoting NSCLC cell lines proliferation and survival mediated by PPARβ/δ was analyzed by siRNA and flow cytometer.The function of PPARβ/δ in regulating the express of Cox-2 and vascular endothelial growth factor (VEGF) gene was confirmed by medicine experiment.Results H441 cells had high levels of PPARβ/δ,Cox-2,cPLA2,PGES and PPARc.H358 and H23 cells had intermediate levels of PPARβ/δ.The PPARβ/δ mRNA level was higher in tumor tissues compared to normal tissues.PPARβ/δ had the function of increasing NSCLC proliferation and survival and low level cPGI2 (activate the PPARβ/δ) could improving NSCLC proliferation and viability,prolonging the S cell cycle and shortening the G1 cell cycle.But there nearly was no impact in A549 cells.Decreasing the cell proliferation and viability and promoting apoptosis when knock-down the PPARβ/δ by siRNA and regulating the Cox-2 and vascular endothelial growth factor (VEGF) expression by PPARβ/δ.There was increasing of Cox-2 and VEGF mRNA during the GW501516 (the ligand of PPARβ/δ) treating NSCLC.But,no change was detected in A549 cells.Conclusion Up-expression in NSCLC compared to normal tissues.There are many function of PPARβ/δ in NSCLC including promoting NSCLC proliferation,survival and viability and affecting the Cox-2,VEGF expression.Together,these data suggest that PPARβ/δ might local the central of multiple signaling pathways and control the NSCLC proliferation. Key words: Peroxisome proliferator-activated receptor β/δ; Non-small cell lung cancer; Proliferation; Potential medicine target
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