Abstract
A calmodulin dependent cyclic nucleotide phosphodiesterase is associated with the head and tailpieces of demembranated rat caudal epididymal sperm. The phosphodiesterase was stimulated two-fold in the presence of Ca 2+, while the simultaneous addition of Ca 2+ and calmodulin resulted in a four-fold increase in activity. Ca 2+ stimulation was abolished if demembranated sperm were extracted with EGTA and was recovered upon the addition of exogenous calmodulin. Micromolar levels of Ca 2+ were required for full stimulation. Trifluoperazine inhibited the Ca 2+ stimulated enzyme in a dose dependent manner (ID 50 = 50 μM) but had no effect on the basal phosphodiesterase activity.
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More From: Biochemical and Biophysical Research Communications
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