Function of calcium-rich hydroxyapatite nanoparticles on proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs) in osteoporosis

Abstract

Out study was aimed to assess the function of calcium-rich hydroxyapatite nanoparticles on proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs) in osteoporosis. The structure of calciumrich hydroxyapatite nanoparticles was tested with Transmission electron microscopy (TEM) and cell proliferation was detected with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The activity of Caspase3 was detected with ELISA and content of alkaline phosphatase (AP), osteopontin (OPN) and Runx2 was tested with Western Blot assay. The bone marrow mesenchymal stem cells (BMSCs) proliferation was prompted by calcium-rich hydroxyapatite. Results showed that the activity of ALP was increased, while activity of Caspase3 was reduced. The expression levels of OPN and Runx2 were increased while expression of Bone morphogenetic protein 2 (BMP-2) osteogenesis marker was increased. Moreover, the BMSCs could be differentiated effectively onto osteogenic direction, and proliferation and osteogenic differentiation of BMSCs was prompted effectively by calcium-rich hydroxyapatite. These findings could probably provide a brand-new scheme for clinical treatment of OP.