Abstract
The ongoing outbreaks of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has led to a worldwide pandemic of Coronavirus Disease (COVID-19) in 2019. Nucleic Acid Tests as the current most prevalent method are able to detect the presence of SARS-CoV-2 from infected patients by comparing target viral genome sequences in high sensitivity and accuracy. Three widely applied nucleic acid methods for clinical and research purpose including RT-PCR tests, LAMP and CRISPR-Cas based detection are introduced firstly, followed by the discussion of Antibody Tests, which are ICG and CL immunoassay tests.These two fields of COVID-19 diagnostic methods exhibit some advantages and drawbacks depending on various clinical settings. Antibody test is supplementary and complementary of other diagnostic methods while Nucleic acid tests are overall effectively and rapidly to diagnose infected patients for immediate treatment and isolation. The combination of these two methods may eventually control the dissemination of COVID-19 pandemic.
Highlights
Introduction should use the fluorescence quantitativePCR instrument specified in the approved product specification
1.1 Nucleic Acid Tests and Serology/Antibody Tests In December 2019, an outbreak of pneumonia with unknown cause began in China's Hubei province, which caused global health problems due to its ease of transmission
Nucleic acid testing is the primary method of diagnosing COVID-19, which offers the most sensitive and early detection of COVID-19
Summary
1.1 Nucleic Acid Tests and Serology/Antibody Tests In December 2019, an outbreak of pneumonia with unknown cause began in China's Hubei province, which caused global health problems due to its ease of transmission. The nucleic acid test is to look for the presence of a novel corona virus in the respiratory tract specimen, blood or stool of a patient to determine whether the patient has been infected with a virus. Several commonly used nucleic acid detection methods are introduced, including Real-Time RT-PCR tests, LAMP and CRISPR-Cas based detection. Molecular diagnosis of the viral genome and serological blood tests are performed by RT-PCR, chest X-ray or CT scan. Nucleic-acid-based tests currently offer the most sensitive and early detection of COVID-19. Several commonly used nucleic acid detection methods are introduced in the following part, including RT-PCR, LAMP, and CRISPR-Cas based detection. 2.1 Real-Time RT-PCR These tests utilize standard RT-PCR protocols, including cell lysis, nucleic acid extraction and purification, and multiple PCR amplification and detection with fluorescence signal readings. Many of the "suspicious" cases have been reported to have typical clinical features of COVID-19 and the same specific computed tomography (CT) images [25]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
