Abstract
BackgroundAccumulated evidences indicate that long non‐coding RNAs (lncRNAs) participate in many biological mechanisms. Moreover, it acts as an essential regulator in various human diseases such as gastric cancer (GC). Nevertheless, the comprehensive regulatory roles and clinical significance of most lncRNAs in GC are not fully understood.MethodsIn this research, our aim was to investigate the underlying mechanism of lncRNA LINC01234 in GC. Firstly, the usage of qRT‐PCR helped to establish expression pattern of LINC01234 in GC tissues. Following this, appropriate statistical tests were applied to analyze the relation between expression level and clinicopathological factors. Ultimately, potential functions and regulatory network of LINC01234 were concluded via GSEA and a series of bioinformatics tools or databases, respectively.ResultsConsequently, at the end of research we found LINC01234 is up‐regulated in GC tissues in comparison with adjacent normal tissues. Furthermore, its expression level is correlated with differentiation of patients with GC. It is also important to highlight bioinformatics analysis revealed that LINC01234 is involved in cancer‐associated pathways such as cell cycle and mismatch repair. Also, regulatory network of LINC01234 presented a probability in the involvement of tumorigenesis through regulating cancer‐associated genes.ConclusionOverall, our results suggested that LINC01234 may play a crucial role in GC.
Highlights
Gastric cancer (GC) is a complex disease caused by accumulation of both genetic and epigenetic factors and imposes a considerable global health burden.[1]
The area under the receiver operating characteristic (ROC) curve (AUC) was 0.888 for The Cancer Genome Atlas (TCGA) dataset while 0.664 for our quantitative reverse transcription-polymerase chain reaction (qRT-PCR) results, indicating that LINC01234 plays a prominent role in gastric cancer (GC) tumorigenesis
An increasing number of long non-coding RNA (lncRNA) have been identified to be related to numerous kinds of diseases,[37] including GC
Summary
Gastric cancer (GC) is a complex disease caused by accumulation of both genetic and epigenetic factors and imposes a considerable global health burden.[1]. Numerous experimental researches have identified several lncRNAs that play crucial role in GC such as imprinted maternally expressed transcript (H19),[13] small nucleolar RNA host gene 5 (SNHG5),[14] homeobox transcript antisense RNA (HOTAIR),[15] AGAP2 antisense RNA 1 (AGAP2-AS1),[16] and Pvt[1] oncogene (PVT1).[17]. They were only a tip of iceberg, there remain a large number of lncRNAs with unknown functions and regulation mechanism in GC. We first analyzed gene expression profiles of STAD patients in TCGA and found a number of lncRNAs differently expressed in cancerous tissues compared with adjacent non-cancerous tissues. Gene Set Enrichment Analysis (GSEA) method and constructed the LINC01234 regulatory network to well interpret the regulation mechanism of LINC01234 in GC
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