Fumigant Toxicity and Repellency Effect of Orange Leaves Citrus sinensis (L.) Essential Oil on Rhyzopertha dominica and Lasioderma serricorne

Abstract

Recently, essential oils have received much attention as pest control agent because of their insecticidal properties, antifeedant and repellent activity. Fumigant toxicity and repellency effect of essential oil of orange leaves Citrus sinensis (L.) were tested on adults of two coleopteran stored product pests, lesser grain borer Rhyzopertha dominica, and cigarette beetle Lasioderma serricorne. The essential oil of orange leaves was obtained by Clevenger-type apparatus. The bioassays were conducted by using 40 ml glass vials as container and Whatman filter paper (1×2 cm) as the essential oil release source. Repellency effect of four concentrations 0.1, 0.2, 0.41 and 0.83 μl/cm2of the essential oil was evaluated by comparing the number of adults in both treated and non-treated portions. Based on probit analysis of data, the LC50 valued were 90.15 and 235/75 μl/l air respectively after 24 h post-treatments. The adult of lesser grain borer were more sensitive than cigarette beetle to this oil. Average repellency, were 49.99 and 58.33% respectively, at three hours post-treatments which are grades of III consisting of the sextet classification.