Abstract

The sensitivity and stability of dehydrogenase-based carbon paste biosensors are greatly improved upon adding fumed-silica nanoparticles into the electrode matrix. The enhanced performance is attributed to improved retention of the dehydrogenases and their NAD + cofactro on the silica support, and to an electrocatalytic action of fumed silica towards the detection of NADH. Such advantages of the silica modified carbon paste are illustrated with the enzymes alcohol and lactate dehydrogenase, and using batch and flow conditions. The response is characterized wih respect to various experimental variables.

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