FULLY INTEGRATED MICROFLUIDIC BIOCHIPS FOR DNA ANALYSIS

Abstract

International Journal of Computational Engineering ScienceVol. 04, No. 02, pp. 145-150 (2003) Invited PaperNo AccessFULLY INTEGRATED MICROFLUIDIC BIOCHIPS FOR DNA ANALYSISROBIN H. LIU, JIANING YANG, RALF LENIGK, JUSTIN BONANNO, FREDERIC ZENHAUSERN, and PIOTR GRODZINSKIROBIN H. LIUArizona State University, Applied NanoBioscience Center, ECA300, MC4004, Tempe, AZ 85287, USA Search for more papers by this author , JIANING YANGArizona State University, Applied NanoBioscience Center, ECA300, MC4004, Tempe, AZ 85287, USA Search for more papers by this author , RALF LENIGKArizona State University, Applied NanoBioscience Center, ECA300, MC4004, Tempe, AZ 85287, USA Search for more papers by this author , JUSTIN BONANNOArizona State University, Applied NanoBioscience Center, ECA300, MC4004, Tempe, AZ 85287, USA Search for more papers by this author , FREDERIC ZENHAUSERNArizona State University, Applied NanoBioscience Center, ECA300, MC4004, Tempe, AZ 85287, USA Search for more papers by this author , and PIOTR GRODZINSKIArizona State University, Applied NanoBioscience Center, ECA300, MC4004, Tempe, AZ 85287, USAPresent address: Alamos National Laboratory, Los Alamos, New Mexico 87545, USA. Search for more papers by this author https://doi.org/10.1142/S1465876303000818Cited by:2 PreviousNext AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsRecommend to Library ShareShare onFacebookTwitterLinked InRedditEmail AbstractMicrofluidics-based biochip devices are developed to perform DNA analysis from complex biological sample solutions. Microfluidic mixers, valves, pumps, channels, chambers, heaters, and DNA microarray sensor are integrated to perform magnetic bead-based rare cell capture, cell preconcentration and purification, cell lysis, polymerase chain reaction, DNA hybridization and electrochemical detection in a single, fully automated biochip device. No external pressure sources, mechanical pumps, or valves are necessary for fluid manipulation, thus eliminating sample contamination and simplifying device operation. Pathogenic bacteria detection and single-nucleotide polymorphism analysis directly from blood are demonstrated. The device with capability of on-chip sample preparation and DNA detection provides a cost-effective solution to direct sample-to-answer genetic analysis, and thus has potential impact in the fields of point-of-care genetic analysis and disease diagnosis.Keywords:Microfluidic BiochipsDNA AnalysisMixingMicrovalves References P. K. Yuenet al., Genome Research 11, 405 (2001). Crossref, Google ScholarJ. Chenget al., Nature Biotechnology 16, 541 (1998). Crossref, Google ScholarR. C. Andersonet al., Nucleic Acid Research 28(12), e60: i (2000). Crossref, Google ScholarM. A. Burnset al., Science 282, 484 (1998). Crossref, Google ScholarS. R. Quake and A. Scherer, Science 290, 1536 (2000). Crossref, Google ScholarD. J. Beebeet al., Nature 404, 588 (2000). Crossref, Google ScholarR. H. Liu, Q. Yu and D. J. Beebe, Journal of Microelectromechanical System 11, 45 (2002). Crossref, Google Scholar M. J. Zdeblick, R. Anderson, J. Jankowski, B. Kline-Schoder, L. Christel, R. Miles and W. Weber, Thermopneumatically actuated microvalves and integrated electro-fluidic circuits. in IEEE Sloid-State Sensors and Actuator Workshop. 1994. Hilton Head, SC . Google ScholarR. M. Umeket al., J. Mol. Diagn. 3, 74 (2001). Crossref, Google ScholarS. A. Elder, J. Acoust. Soc. Am. 31, 54 (1959). Crossref, Google Scholar G. T. A. Kovacs , Micromachined Transducers Sourcebook ( WCB McGraw-Hill , Boston , 1998 ) . Google Scholar FiguresReferencesRelatedDetailsCited By 2Optimization of direct whole blood PCR amplification with applications on a static thermostat chipBai-Yan Qu, Zhi-Yong Wu, Xiao-Xi Tian, Kun Chen and Fang Fang12 September 2007 | Analytical and Bioanalytical Chemistry, Vol. 389, No. 5Microfluidic sample preparation for nucleic acid analysisV. Samper and Q. Ramadan Recommended Vol. 04, No. 02 Metrics History KeywordsMicrofluidic BiochipsDNA AnalysisMixingMicrovalvesPDF download