Abstract
A novel cartridge-based platform was developed to perform fully automated sample preparation, amplification, and analysis of nucleic acids. The cartridge consists of three layers: multiple reagent chambers, a mixing reservoir, and elutes and waste chambers. The fluidic manipulation and solution mixing inside the cartridge were controlled by motor systems. After loading a sample specimen, each reagent solution in the chambers of the cartridge was sequentially injected into the mixing reservoir. Extracted nucleic acids were captured by magnetic beads and transferred into a small eluates chamber. Automated sample preparation and isothermal amplification of nucleic acids for pathogenic bacteria were demonstrated in this all-in one cartridge platform. This cartridge system provides rapid and reproducible sample preparation in comparison with manual methods. In addition, real-time detection of isothermal amplification using the implemented photometric system was compared with that of using a commercial polymerase chain reaction (PCR) device. This system has the potential to become a more reliable diagnostic tool for fast detection of contagious, foodborne, and emergency point-of-care (POC) diseases.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Precision Engineering and Manufacturing
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
