Abstract
Fast and fully automated deoxyribonucleic acid (DNA) amplification methods are of interest in the research on lab-on-a-disc (LOD) platforms because of their full compatibility with the spin-column mechanism using centrifugal force. However, the standard procedures followed in DNA amplification require accurate noncontact temperature control as well as cell lysis at a low temperature to prevent damage to the LOD platform. This requirement makes it challenging to achieve full automation of DNA amplification on an LOD. In this paper, a fully automated LOD capable of performing cell lysis and amplification on a single compact disc of DNA samples is proposed. The proposed system uses micro-carbon to heat DNA samples without damaging the LOD as well as a noncontact heating system and an infrared camera sensor to remotely measure the real temperature of the amplification chamber. Compared with conventional DNA amplification systems, the proposed system has the advantage of full automation of the LOD platform. Experimental results demonstrated that the proposed system offers a stable heating method for DNA amplification and cell lysis.
Highlights
Over the past few decades, the miniaturization and automation of microfluidics-based analysis protocols have accelerated the development of integrated and low-cost point-of-care (POC) devices for rapid diagnosis
Graphite is a substance with a high heat of fusion, melting, and solidification aThe certain additionontothe implementing cell lysis using micro-carbon-activated heatingstudy
The leakage problem was efficiently addressed by the internal structural design of the disc
Summary
Over the past few decades, the miniaturization and automation of microfluidics-based analysis protocols have accelerated the development of integrated and low-cost point-of-care (POC) devices for rapid diagnosis. The performance of microfluidics in POC applications is based on the integration of the key operations on a monolithic device. Simple independent microfluidic devices cannot perform the overall bioassay protocol processes, necessitating an integrated microfluidic POC system. As an extension to these systems, a chip design using centrifugal microfluidic technology, which is known as a lab-on-a-disc (LOD) platform, is proposed . An external pump and multiple laboratory instruments are no longer required Another major advantage of these systems is that the centrifugal force alone regulates the fluid control; the overall process can be fully automated. Owing to these advantages, temperature control and heating systems can be applied to LOD platforms. A heat-sink device is often used as the basis for analytical
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